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ngb  (Novus Biologicals)


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    Novus Biologicals ngb
    Ngb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neuroglobin/Neuroglobin+Antibody/pm41513082-90-18-30
    Average 94 stars, based on 1 article reviews
    ngb - by Bioz Stars, 2026-08
    94/100 stars

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    (a) Schematic of the genetic engineering strategy for the generation of the ChuA reporter strain used in this study. (b) The growth phenotype of the strain described in (a), grown on LB agar (top, representative images shown) in the presence of iron-limited LB agar supplemented with either 5 µM αβHb, αγHb, hemin, myoglobin, neuroglobin, cytoglobin or human ferredoxin 1. (below) EC 50 values of E. coli ΔTBDT:ChuOP cultured in LB liquid medium in the presence of 200 µM 2,2’-bipyridine, supplemented with serially diluted αβHb, αγHb, hemin, myoglobin, neuroglobin, cytoglobin or ferredoxin 1.

    Journal: bioRxiv

    Article Title: Inhibiting heme-piracy by pathogenic Escherichia coli using de novo -designed proteins

    doi: 10.1101/2024.12.05.626953

    Figure Lengend Snippet: (a) Schematic of the genetic engineering strategy for the generation of the ChuA reporter strain used in this study. (b) The growth phenotype of the strain described in (a), grown on LB agar (top, representative images shown) in the presence of iron-limited LB agar supplemented with either 5 µM αβHb, αγHb, hemin, myoglobin, neuroglobin, cytoglobin or human ferredoxin 1. (below) EC 50 values of E. coli ΔTBDT:ChuOP cultured in LB liquid medium in the presence of 200 µM 2,2’-bipyridine, supplemented with serially diluted αβHb, αγHb, hemin, myoglobin, neuroglobin, cytoglobin or ferredoxin 1.

    Article Snippet: Sequences for the expression of recombinant human αβ and αγ hemoglobin, myoglobin, neuroglobin, cytoglobin and ferredoxin 1 were ordered as gene fragments from Twist Biosciences and cloned into either pETDuet-1 (for hemoglobin), pET29a or pET22b, carrying an N-terminal 6x His tag.

    Techniques: Cell Culture